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American Society of Microbiology ABMM Sample Questions:

1. A research team is investigating the host immune response to Mycobacterium tuberculosis. They discover that a specific mycobacterial glycolipid inhibits the maturation of phagosomes containing the bacteria, preventing their fusion with lysosomes. This mechanism allows M. tuberculosis to:

A) Induce the production of pro-inflammatory cytokines.
B) Escape from the phagolysosome into the cytoplasm.
C) Evade detection by Toll-like receptors on macrophages.
D) Survive and replicate within macrophages.

2. A patient with a history of heart valve replacement develops subacute bacterial endocarditis. Blood cultures grow a Gram-positive coccus that is alpha-hemolytic on blood agar, catalase-negative, and optochin-resistant.
The MOST likely organism is:

A) Viridans streptococci
B) Enterococcus faecalis
C) Streptococcus pneumoniae
D) Streptococcus pyogenes

3. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true positive results divided by the total number of positive test results.
B) The number of true negative results divided by the total number of negative test results.
C) The number of true negative results divided by the total number of true negative and false positive results.
D) The number of true positive results divided by the total number of true positive and false negative results.

4. A patient with a history of liver transplantation develops a bloodstream infection with a Gram-negative bacterium. The isolate is identified as Sphingomonas paucimobilis. This opportunistic pathogen is known for its ability to:

A) Exhibit intrinsic resistance to a broad spectrum of antibiotics.
B) Form biofilms on indwelling catheters and prosthetic devices.
C) Produce potent exotoxins that directly damage host tissues.
D) Degrade a wide range of environmental pollutants.

5. A microbiology laboratory isolates a Gram-negative rod from a wound infection. The organism is oxidase- negative, ferments lactose on MacConkey agar, and produces indole. The MOST likely identification is:

A) Serratia marcescens
B) Escherichia coli
C) Proteus mirabilis
D) Klebsiella pneumoniae

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